Vial Reconstitution

Reconstitution Calculator

Pick a vial, set the water volume, enter your target dose. The output is the exact syringe line to draw to. For in-vitro research use only.

What you'll need

  • Your peptide vial — the freeze-dried powder you ordered (it looks like a tiny cake or fluff at the bottom of a small glass vial).
  • Bacteriostatic water — sterile water with 0.9% benzyl alcohol that lets you draw multiple doses over 28 days. Sold as a 3mL vial. Don't use tap water or saline.
  • U-100 insulin syringes — 1mL barrel with 100 small ticks. Standard, cheap, widely available.
  • Alcohol wipes — to clean the rubber stopper before each draw.

How it works

  1. Pick the peptide vial you bought from our shop.
  2. Choose how much bacteriostatic water you'll mix in (1, 2, or 3 mL). Less water = stronger mix, more water = finer dose control.
  3. Enter the dose your research protocol calls for (in mg or mcg).
  4. Read the highlighted line on the syringe diagram — that's the exact tick mark to pull the plunger to.

Your setup

Three inputs. Output updates live.

Each option matches a product. The number is the milligrams in the vial.

2 mL is the standard. Less water = stronger mix. More water = finer dose control.

Tap a chip to autofill a typical protocol dose. Fine-tune after.

Toggle mg / mcg on the right. 1 mg = 1000 mcg.

Research only. Output assumes a U-100 insulin syringe (100 units = 1 mL). Always cross-reference your vial label and a published research protocol before any in-vitro work.

Pull the plunger to this line

5.0units

for 250 mcg dose

Stop the plunger at this tick on a U-100 insulin syringe. Diagram below.

Syringe diagram

0 30 60 90 100 5.0 units

Pull the plunger until its rubber stopper aligns with the 5.0-unit mark.

Your mix strength

5.00mg / mL

10mg vial + 2mL water

Per syringe tick

50.0mcg

1 tick = 1 unit = 0.01 mL

Other doses at this mix

If your dose isThat's this volumePull syringe to

Reconstitution FAQ

What is reconstitution?

Mixing the dry peptide powder in your vial with sterile water so it becomes a liquid you can draw into a syringe. The peptide ships freeze-dried (lyophilized) so it stays stable on the shelf — it only becomes injectable once you add water. Reconstitution is a one-time step you do when you first open the vial.

What is bacteriostatic water? Can I use tap water or saline?

Bacteriostatic water is sterile water that contains 0.9% benzyl alcohol — a preservative that prevents bacteria from growing in the vial after you open it. That's important because peptide vials are multi-dose: you draw from the same vial over 28+ days. Tap water and saline are not interchangeable. Don't substitute either. We sell 3 mL bacteriostatic water vials on the shop.

What's a U-100 insulin syringe?

A 1 mL syringe with 100 small tick marks on the barrel. Each tick = 1 unit = 0.01 mL. They're cheap, sold at most pharmacies, and the standard tool for peptide research because the small ticks let you draw very precise doses. The "U-100" just means there are 100 units of marking per millilitre.

What's the difference between mg and mcg?

1 mg = 1000 mcg. Most repair-style peptides (BPC-157, TB-500, KPV, GHK-Cu) are dosed in micrograms (mcg) — research protocols typically use 250–500 mcg per injection. GLP-1 peptides (retatrutide, semaglutide) are dosed in milligrams (mg) — typically 1–6 mg per injection. The calculator handles both — just toggle the unit on the right side of the dose input.

Which water volume should I pick — 1, 2, or 3 mL?

Most published BPC, TB, and GHK-Cu protocols reconstitute at 2 mL — it's the safest default and gives clean syringe-tick numbers for typical doses. Pick 1 mL for a stronger mix (good if your dose is large and you want fewer units to draw). Pick 3 mL for finer dose control on very small doses (like CJC + Ipa). The vial size doesn't change — only how concentrated the liquid becomes.

How long does a reconstituted vial last?

Bacteriostatic water's 0.9% benzyl alcohol typically supports a 28-day window when the reconstituted vial is refrigerated at 2–8°C (regular fridge temperature). Stability varies a bit by peptide — see the peptide stability and storage guide for compound-specific data.

How do I physically reconstitute the vial? (step by step)

1. Wipe the rubber stopper of both the peptide vial and the bacteriostatic water vial with an alcohol wipe. 2. Use a syringe to draw the amount of bacteriostatic water shown above (look at "Your mix strength" — if you used 2 mL water, draw 200 units / 2 mL). 3. Insert the needle into the peptide vial at an angle and inject the water slowly down the inside wall of the vial. Don't squirt directly onto the powder. 4. Swirl gently — never shake — until the powder fully dissolves into a clear liquid. 5. Refrigerate. Each subsequent dose: wipe the stopper, draw to the line shown in the syringe diagram above, inject.

How is the math done?

Concentration (mg/mL) = peptide milligrams in the vial ÷ millilitres of bacteriostatic water. Units to draw = (target dose in mg ÷ concentration) × 100. Example: 10 mg BPC-157 + 2 mL water = 5 mg/mL. For a 250 mcg (0.25 mg) dose: (0.25 ÷ 5) × 100 = 5 units on the syringe.

Why does the calculator default to a U-100 insulin syringe?

U-100 syringes are the standard format used in published research protocols. They mark 100 units per millilitre, so 1 unit equals 0.01 mL regardless of which peptide is loaded. The unit count is therefore directly comparable across reconstitution volumes.

How do I read the output for blend vials like GLOW or BPC + TB?

Blend vials report the combined milligrams across all actives — GLOW is 70mg total (50mg GHK-Cu + 10mg BPC-157 + 10mg TB-500), BPC + TB is 10mg total (5mg + 5mg). The "units to draw" output reflects the blend as a single unit, and each draw delivers the components in their native ratio.

Why isn't HCG in this calculator?

HCG is dosed in international units (IU), not milligrams, so the mg/mL math does not apply directly. A separate HCG calculator with IU-based reconstitution math will be added later. For now, refer to the HCG product page for vial-specific reconstitution notes.

Where can I verify the peptide content of a vial?

Every Remy Peptides vial ships with batch ID matching a published Janoshik HPLC certificate. Open the COA library to look up a specific batch, or read how to verify a Janoshik COA for a step-by-step walkthrough.